Study on Resveratrol Chemoprotective Effect and COX 2 Down Regulation in Tumor Suppression in DMBA Induced Breast Cancer in Female Sprague Dawley Rats
Background: The purpose of this study was to see if Resveratrol might help protect female Sprague Dawley rats from developing breast cancer caused by 7,12 Dimethylbenzanthracene (DMBA), as well as if it could help downregulate COX 2, an enzyme seen in breast cancer tissues. Methods: In this investigation, 40 female Sprague Dawley rats (n = 10 per group) were employed. 6 week old rats were divided into four groups: group 1 received pulverised rodent diet, group 2 received DMBA and diet, group 3 received DMBA and diet with Resveratrol 100mcg, and group 4 received DMBA and diet with Resveratrol 100mcg. 200mcg. After 120 days, the study was terminated, and tumours were assessed for multiplicity, incidence, and histology. Cox 2 expression was examined by Western blot analysis. One way variance and Tukey’s comparison test were used to test the values statistically. Body weight and tumour volume were similar in the resveratrol-treated groups, and there was a very lengthy latency time for tumour initiation, as well as a reduction in tumour multiplicity and incidence. Group 2 had a tumour incidence of 42.2710.17, Group 3 had a tumour incidence of 21.915.87, and Group 4 had a tumour incidence of 13.733.98. Group 2 had a tumour multiplicity of 0.89090.30, group 3 had a tumour multiplicity of 0.10360.04, and group 4 had a tumour multiplicity of 0.045450.02. In groups 2, 3, and 4, histopathological investigation indicated ductal carcinoma, mild tissue necrosis, and fibroadenoma. Resveratrol reduces COX 2 expression in breast carcinoma and has a chemopreventive impact against DMBA-induced breast cancer, according to the findings.
Dr. Aneena Suresh
Department of Pharmacy Practice, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, India.
Department of Pharmacy Practice, Asmara College of Health Sciences, Asmara, Eritrea.
Department of Pharmacy Practice, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Ooty, Nilgiris, Tamil Nadu, India.
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