Study on Isolation, Characterization and Identification of Endophytic Bacteria in Maize (Zea mays L.) Cultivated on Acrisols of the Southeast of Vietnam

The variety of endophytic bacteria in maize (Zea mays L.) grown on Acrisols in Southeast Vietnam was investigated. In this region, twenty-eight maize stem and root samples were gathered from five different locations (provinces/cities). Endophytic bacteria were isolated in two mediums (LGI and NFb) and 16S rRNA gene fragments were amplified from DNA using eubacterial universal primers (p515FPL and p13B). A total of 301 isolates were isolated, and all of them were capable of nitrogen fixation, phosphate solubilization, and IAA biosynthesis, but 30 isolates stood out, and they were recognised as maize endophytes and nifH gene owners. The genomes of 30 endophytic bacteria isolates were found to have a high degree of similarity to the GenBank reference strains (between 97 percent and 100 percent ). Six isolates belonged to Bacillus (20.00%), and 24 isolates belonged to Proteobacteria (80.00%), including two alpha-proteobacteria (7%), six beta-proteobacteria (20%), and sixteen gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-proteobacteria (16 gamma-p (53 percent ). Proteobacteria had the highest Theta values based on Pi value (nucleotide diversity), and Theta values (per sequence) from S of SNP for DNA polymorphism were computed for each group, with Proteobacteria having the highest values in comparison to two groups. Because of their usefulness and biosafety, three isolates, DTN1b (Azotobacter vinelandii), VTN2b (Bacillus subtilis), and VTN7 (Enterobacter cloacae), have been recommended as suitable microbial inoculants or biofertilizers for sustainable maize cultivation in low Acrisols in Vietnam.

Author(S) Details

Dang Thi Ngoc Thanh
Department of Natural Science Teacher Training, Sai Gon University, HCM City, Vietnam.

Cao Ngoc Diep
Department of Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam.

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