News Update on Rice Cultivar Research: July – 2019

RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar.

Moroberekan, a japonica rice vascular plant with sturdy resistance to blast unwellness in Asia, was crossed to the extremely inclined indica vascular plant, CO39, and 281 F7 recombinant inbred (RI) lines were created by single seed descent. The population was evaluated for blast resistance within the greenhouse and also the field, and was analyzed with 127 fragment length polymorphism (RFLP) markers. 2 dominant loci related to qualitative resistance to 5 isolates of the plant life were tentatively named Pi-5(t) and Pi-7(t). They were mapped on chromosomes four and eleven, severally. to spot quantitative attribute loci (QTLs) poignant partial resistance, Rhode Island lines were inoculated with isolate PO6-6 of Pyricularia oryzae in polycyclic tests. 10 body segments were found to be related to effects on lesion range (P < zero.0001 and LOD > half-dozen.0). 3 of the markers related to QTLs for partial resistance had been reportable to be joined to finish blast resistance in previous studies. QTLs known in greenhouse tests were sensible predictors of blast resistance at 2 field sites. This study illustrates the utility of Rhode Island lines for mapping a posh attribute like blast resistance and suggests that sturdy resistance within the ancient selection, Moroberekan, involves a posh of genes related to each partial and complete resistance. [1]

Construction and Evaluation of Chromosome Segment Substitution Lines Carrying Overlapping Chromosome Segments of indica Rice Cultivar ‘Kasalath’ in a Genetic Background of japonica Elite Culture ‘Koshihikari’

To Facilitate The Genetic Analysis Of Quantitative Traits and therefore the Use Of Marker-Assisted Breeding In Rice, we tend to Developed a completely unique Mapping Population Consisting Of thirty-nine body phase Substitution Lines (CSSLs). In every Line, a distinct body phase Of The Indica tracheophyte ‘Kasalath ‘was substituted within the genetic background of the japonicaThe substituted chromosomal segments in the thirty-nine CSSLs coated most of the ordering, aside from tiny regions at the distal finish of the short arm of chromosomes eight and at the distal end of the long arm of chromosomes tracheophyte ‘Koshihikari’ (Japanese elite cultivar). 12. To verify the potential blessings of quantitative loci (QTL) detection in these CSSLs, we tend to used the CSSLs to find QTLs for heading date below 3 completely different environmental conditions: a natural summer field in Tsukuba, Japan -h light), and short-day conditions (10-h light). The results clearly incontestible that the employment of CSSLs enabled to spot a bigger variety of QTLs than did a before Christ one F three we tend to conducted many blessings of the use of CSSLs in terms of genetic analysis, molecular biological research of QTLs, and marker-assisted choice in rice breeding. population derived from constant cross combination. [2]

Rice cultivar evaluation for phosphorus use efficiency

Phosphorus deficiency is one in every of the foremost growth-limiting factors in acid soils in numerous elements of the planet. the target of this study was to screen twenty five rice cultivars (Oryza sativa L.) at low, medium, and high levels of soil P. range of tillers, root length, plant height, root dry weight and shoot dry weight were associated with tissue P concentrations, P uptake and P-use potency. Shoot weight was found to be the plant parameter most sensitive to P deficiency. important tracheophyte variations in P use potency were found. Phosphorus use potency was higher in roots than shoots and diminished with increasing levels of soil P. Positive correlations were found among growth parameters like plant height, tillers, root and shoot weight, and P content of roots and shoots. These results indicate choice of rice cultivars for satisfactory performance underneath low P handiness is disbursed mistreatment shoot and root dry weight as criteria. [3]

2,4-D attenuates salinity-induced toxicity by mediating anatomical changes, antioxidant capacity and cation transporters in the roots of rice cultivars

Growth regulator herbicides are wide employed in paddy fields to manage weeds, but their role in conferring environmental stress tolerance within the crop plants are still elusive. during this study, the results of suggested dose of two,4-dichlorophenoxyacetic acid (2,4-D)  on growth, aerophilic harm, inhibitor defense, regulation of ion transporter genes and anatomical changes within the roots of rice cultivars XS 134 (salt resistant) and ZJ eighty eight (salt sensitive) were investigated below completely different levels of saline stress. Individual treatments of saline stress and a couple of,4-D application iatrogenic aerophilic harm as proved  by cut root growth, increased ROS production, additional membrane harm and Na+ accumulation in sensitive vascular plant compared to the tolerant cultivar. Conversely, combined treatments of two,4-D and saline stress considerably mitigated the expansion inhibition and aerophilic stress in roots of rice cultivars by modulating polymer and callose deposition, oxidoreduction states of AsA, GSH, and connected accelerator activities concerned within the inhibitor weapons system. The expression analysis of 9 ion transporter genes showed altered and differential organic phenomenon in salt-stressed roots of sensitive and resistant cultivars. Together, these results recommend that a pair of,4-D differentially regulates the Na+ and K+ levels, ROS production, inhibitor defense, anatomical changes and ion transporters/genes in roots of rice cultivars. [4]

Root Iron Localization and Proteomic Impairment in Anaerobic Rice Cultivars Exposed to Excess Iron

Plant faces iron (Fe) toxicity once the concentration of this mineral is high in soils below anaerobic conditions. Excess soil Fe2+may cause severe impairment in rice photosynthe­sis, morphological parameters, and should induce aerophilic harm with important alteration in macromolecule profiles. gift study aimed to analyze the extension of aerophilic stress on exposure to excess Fe2+ particle and its effects on the speed of chemical change and modifications in macromolecule profiles of rice cultivars with differential sensitivity. A pot experiment was conducted with 3 Sali rice cultivars, one standard tolerant tracheophyte Mahsuri and midiron sensitive varieties Siyal Sali and Ranjit. Constant waterlog atmosphere with four completely different Fe2+ doses +100 ppm, +200 ppm and +300 ppm treatments and an effect while not external Fe, were dead. Mahsuri displayed well adaptation to bronzed diabetes recording superior morphological parameters with higher chemical action activity compared to Fe intolerant varieties. The SDS-PAGE results of leaf macromolecule showed that Mahsuri had larger numbers of intense bands indicating a lot of leaf proteins accumulation all told treatments. In distinction Ranjit and Siyal Sali expressed solely few weak bands once supplemented with higher Fe2+ doses. therefore proteomic comparison between tolerant and sensitive cultivars once bronzed diabetes provides insight into the transcriptional regulation of the variability to tolerance response. These findings establish the foundations of introducing iron tolerance into farmers’ friendly rice cultivars triggering higher nutritionary values. [5]


[1] Wang, G.L., Mackill, D.J., Bonman, J.M., McCouch, S.R., Champoux, M.C. and Nelson, R.J., 1994. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics, 136(4), pp.1421-1434. (Web Link)

[2] Ebitani, T., Takeuchi, Y., Nonoue, Y., Yamamoto, T., Takeuchi, K. and Yano, M., 2005. Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar ‘Kasalath’in a genetic background of japonica elite cultivar ‘Koshihikari’. Breeding Science, 55(1), pp.65-73. (Web Link)

[3] Fageria, N.K., Wright, R.J. and Baligar, V.C., 1988. Rice cultivar evaluation for phosphorus use efficiency. Plant and Soil, 111(1), pp.105-109. (Web Link)

[4] 2,4-D attenuates salinity-induced toxicity by mediating anatomical changes, antioxidant capacity and cation transporters in the roots of rice cultivars

Faisal Islam, Muhammad A. Farooq, Rafaqat A. Gill, Jian Wang, Chong Yang, Basharat Ali, Guang-Xi Wang & Weijun Zhou

Scientific Reportsvolume 7, Article number: 10443 (2017) (Web Link)

[5] Saikia, T., Stafford, R., Bhuyan, J. and Borthakur, A. (2019) “Root Iron Localization and Proteomic Impairment in Anaerobic Rice Cultivars Exposed to Excess Iron”, International Journal of Plant & Soil Science, 26(1), pp. 1-9. doi: 10.9734/IJPSS/2018/46148. (Web Link)

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