News Update on Cryobiology Research: April – 2019
Three-dimensional printing can provide customizable probes for sensing and monitoring in cryobiology applications
Cryopreservation has been recognized as a robust tool for long-run preservation of genetic resources. However, the outcomes of cryopreservation by totally different user teams typically vary thanks to inconsistency in procedures and freeze instrumentality. Herein, we have a tendency to report on the feasibleness of providing customizable sensing probes with three-dimensional (3-D) printing to watch cryopreservation phenomena. The objectives were to: 1) introduce 3D printing as a fabrication technique for developing customizable probes to be employed in refrigerant applications; 2) style Associate in Nursingd fabricate an example of a 3-D written sensing probe and electronic device capable of detection phase-change phenomena supported quantitative knowledge relating to sample electrical phenomenon and temperature, and 3) demonstrate the sensing platform in cryopreservation conditions and together with a tailor-made 3D written freeze device. The sensing probe developed was designed to suit among normal zero.5-ml French straws. Phase-transition phenomena were detected by analyzing electrical phenomenon changes. The quantitative knowledge from this device in conjugation with a 3D written Deepfreeze rack provided cryopreservation capability with high duplicability and offered an alternate to dear programmable freezers. the utilization of 3D printing provided flexibility to develop new sensing probes or modify existing styles supported specific desires. once initial prototyping, fabrication, and testing of 3D written sensing probes, notably helpful styles will cause the reduction of variation in acting standardized cryopreservation protocols. Cryopreservation has been recognized as a robust tool for long-run preservation of genetic resources. However, the outcomes of cryopreservation by totally different user teams typically vary thanks to inconsistency in procedures and freeze instrumentality. Herein, we have a tendency to report on the feasibleness of providing customizable sensing probes with three-dimensional (3-D) printing to watch cryopreservation phenomena. The objectives were to: 1) introduce 3D printing as a fabrication technique for developing customizable probes to be employed in refrigerant applications; 2) style Associate in Nursingd fabricate an example of a 3-D written sensing probe and electronic device capable of detection phase-change phenomena supported quantitative knowledge relating to sample electrical phenomenon and temperature, and 3) demonstrate the sensing platform in cryopreservation conditions and together with a tailor-made 3D written freeze device. The sensing probe developed was designed to suit among normal zero.5-ml French straws. Phase-transition phenomena were detected by analyzing electrical phenomenon changes. The quantitative knowledge from this device in conjugation with a 3D written Deepfreeze rack provided cryopreservation capability with high duplicability and offered an alternate to dear programmable freezers. the utilization of 3D printing provided flexibility to develop new sensing probes or modify existing styles supported specific desires. once initial prototyping, fabrication, and testing of 3D written sensing probes, notably helpful styles will cause the reduction of variation in acting standardized cryopreservation protocols. [1]
Effects of four disaccharides on nucleation and growth of ice crystals in concentrated glycerol aqueous solution
Devitrification has been determined to be one in every of the main causes of death in cryopreservation by vitrification technique. Reliable quantification of the nucleation and growth of ice crystals of devitrification is of nice importance for the improvement of the vitrification solutions. within the gift study, cryomicroscopy was accustomed investigate the nucleation and growth of ice crystals in targeted alcohol solution (60 wt%) within the presence of saccharose, trehalose, disaccharide and disaccharide. Results showed that saccharose instead of trehalose looks to be the foremost effective one to inhibit the nucleation and ice growth, despite the superb repressing ability of trehalose on ice growth that has been confirmed in several researches. Hence, for ice inhibition, saccharose was a more practical disaccharide additive to suppress nucleation and growth of ice crystals that occurred throughout devitrification in targeted alcohol solutions. [2]
Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders
The aim of this study was to judge totally different concentrations of secretion (RJ) supplemented extenders for post-thawing quality of drone gamete. seed samples were collected from sexually mature drones. Pooled seed was diluted with extender while not RJ (control) or supplemented with totally different concentrations of RJ (1, 2, 4 or 8%). gamete motility, semipermeable membrane practical integrity, and acrosomal integrity were evaluated. At post thaw, the best gamete motility and acrosomal integrity rates were obtained within the RJ1 cluster. practical integrity of gamete membrane was higher preserved in
the RJ1 and RJ2 teams compare to the opposite groups. The study shows that RJ supplemented extenders have useful effects on drone seed parameters. The results of this study incontestable advantage of mistreatment one hundred forty five RJ supplemented extender. [3]
Long-term Preservation of Bone Marrow
PRESERVATION of the practical integrity of cells hold on at low temperatures has become habitually productive. Current efforts in biological science centre around improvement of the techniques, assessment of the physico-chemical alteration of cells by low temperatures, and analysis of the length of your time that frozen cells can maintain their practical capability. it’s been reported , as an example, that bull spermatozoa is hold on with success at − seven9° C for a minimum of 7 years1, which some human cell lines stay viable for thirty three months2. throughout the course of experiments with varied techniques for state change and storing mouse bone marrow3, we have a tendency to froze variety of samples to be tested at long intervals. this interim report describes the results of those experiments. [4]
The Passage Effect on the Senescence Profile of Cryopreserved Bone Marrow and Adipose-Derived Mesenchymal Stem Cells
Background: though bone marrow is the ‘gold standard’ MSc supply, fat has become a promising various supply. Passage and cryopreservation are 2 effective strategies to multiply, pool, and store MSc while not sterilization its perform
Aims: to analyze the passage effects on the senescence profile of cryopreserved bone marrow and adipose-derived mesenchymal stem cells (MSCs).
Study Design: Analytical empirical study.
Place and period of Study: somatic cell Medical Technology Integrated Service Unit, school of drugs, Universitas Indonesia—Cipto Mangunkusumo Hospital, Jakarta, Indonesia, throughout the amount of Gregorian calendar month to Sep 2016.
Methodology: we tend to analyzed the viability, cell size, population doubling time (PDT), proportion of aging cells, and colony forming unit. Samples were bone marrow and fatty MSCs at passage one, that was cryopreserved for the primary and second time. Numerical knowledge were analyzed exploitation the Student’s T check and analysis of variance (ANOVA) test.
Results: each in once and doubly cryopreservation cluster, PDT and aging cell proportion of bone marrow and fat MSCs differed considerably, wherever the PDT aging cell proportion values of bone marrow MSCs were higher altogether passages compared to fat. relating to thirtieth confluence cell size and viability, vital variations between once and doubly cryopreservation cluster varied and didn’t show any trend. The cell size and viability were less 2500 µm2, and quite eighty five, severally. Therefore, the distinction in cell size at thirtieth confluence and viability may well be thought to be traditional variations.
Conclusion: Cryopreserved adipose-derived MSCs showed higher results compared to cryopreserved bone marrow-derived MSCs in terms of population doubling time (PDT) and senescence. [5]
Reference
[1] Shamkhalichenar, H., Choi, J.W. and Tiersch, T.R., 2019. Three-dimensional printing can provide customizable probes for sensing and monitoring in cryobiology applications. Cryobiology. (Web Link)
[2] Zhang, M., Gao, C., Ye, B., Tang, J. and Jiang, B., 2019. Effects of four disaccharides on nucleation and growth of ice crystals in concentrated glycerol aqueous solution. Cryobiology, 86, pp.47-51. (Web Link)
[3] Alcay, S., Cakmak, S., Cakmak, I., Mulkpinar, E., Gokce, E., Ustuner, B., Sen, H. and Nur, Z., 2019. Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders. Cryobiology. (Web Link)
[4] Long-term Preservation of Bone Marrow
L. H. SMITH & PHAN THE TRAN
Naturevolume 205, pages503–504 (1965) (Web Link)
[5] Ismail, H. D., Arif, S., Pawitan, J. A. and Anggraeni, R. (2018) “The Passage Effect on the Senescence Profile of Cryopreserved Bone Marrow and Adipose-Derived Mesenchymal Stem Cells”, Annual Research & Review in Biology, 24(1), pp. 1-11. doi: 10.9734/ARRB/2018/39183 (Web Link)