Determination of Immunological Characterization and Verification of Recombinant Streptococcal Protein G | Chapter 2 | New Visions in Biological Science Vol.10

The purpose of this study was to identify the immunological characterisation and verification of recombinant streptococcal protein G (rSPG), which is essential since it has a higher specificity for immunoglobulin (Ig) than staphylococcal protein A. However, the high expense of commercial recombinant (r) SPG has stymied further study into rSPG’s applicability. The immunological characterization of purified rSPG was compared to commercial SPG using western blot analysis in this study, which was accomplished through effective high cell density fermentation of genetically modified Escherichia coli in earlier work. Purified rSPG had a significantly better IgG-binding capacity than commercial SPG, according to the findings of the current investigation. Purified rSPG cross-linked with Q Sepharose® Fast Flow also had a high affinity for IgG in mouse serum. The purified rSPG was detected using Nanoflow Liquid Chromatography-Mass Spectrometry (MS)/MS spectrum in order to achieve relatively pure and accurate rSPG. The two peptide segments of purified rSPG were found to belong to the Streptococcus sp. GX7805 protein G listed in the National Center for Biotechnology Information database. The approach reported in this paper provides a novel practical method for verifying rSPG in a relatively pure form, which can be used to purify IgG or perform immunolabeling operations.

Author(s) Details:

Hucheng Zhang,
Department of Biology Engineering, Beijing Polytechnic, Beijing-100176, P.R., China.

Weifeng Liang,
Beijing Buchang Pharm Biopharmaceutical R&D Center, Beijing-100080, P.R., China.

Haitao Fan,
Department of Biology Engineering, Beijing Polytechnic, Beijing-100176, P.R., China.

Jun Yang,
Department of Biology Engineering, Beijing Polytechnic, Beijing-100176, P.R., China.

Guowei Yang,
Department of Biology Engineering, Beijing Polytechnic, Beijing-100176, P.R., China.

Xiaojie Wang,
Department of Biology Engineering, Beijing Polytechnic, Beijing-100176, P.R., China.

Liang Chen,
Department of Biology Engineering, Beijing Polytechnic, Beijing-100176, P.R., China.

Tao Liang,
Department of Life Sciences, Tianjin Normal University, Tianjin-300384, P.R. ,China.

Please see the link here: https://stm.bookpi.org/NVBS-V10/article/view/5981