Editor Papa Research April 24, 2019

CRISPR/Cas9 for cancer research and therapy

CRISPR/Cas9 has become a strong methodology for creating changes to the order of the many organisms. 1st discovered in bacterium as a part of Associate in Nursing adaptational system, CRISPR/Cas9 and changed versions have found a widespread use to engineer genomes and to activate or to repress the expression of genes. As such, CRISPR/Cas9 guarantees to accelerate cancer analysis by providing Associate in Nursing economical technology to dissect mechanisms of tumorigenesis, determine targets for drug development, and probably arm cells for cell-based therapies. Here, we tend to review current applications of the CRISPR/Cas9 technology for cancer analysis and medical aid. we tend to describe novel Cas9 variants and the way they’re employed in genomics to get novel cancer-specific vulnerabilities. what is more, we tend to highlight the impact of CRISPR/Cas9 in generating organoid and mouse models of cancer. Finally, we offer an outline of the primary clinical trials that apply CRISPR/Cas9 as a therapeutic approach against cancer. [1]

MicroRNA‐mediated drug resistance in ovarian cancer

The development of intrinsic or noninheritable  resistance to chemotherapeutical agents utilized in the treatment of assorted human cancers may be a major obstacle for the made termination of cancer. The accumulated efforts within the understanding the precise mechanisms of development of multidrug resistance (MDR) have junction rectifier to the introduction of many distinctive and customary mechanisms. Recent studies demonstrate the regulative role of tiny noncoding ribonucleic acid or miRNA within the many elements of cancer biology. much all aspects of cell physiology beneath traditional and illness conditions are according to be controlled by miRNAs. during this review, we have a tendency to discuss however the miRNA profile is modified upon MDR development and also the polar regulative role contend by miRNAs in overcoming resistance to chemotherapeutical agents. it’s hoped that more studies can support the employment of those differentially expressed miRNAs as prognostic and prognostic markers, moreover as novel therapeutic targets to beat resistance in sex gland cancer. [2]

Genome sequencing analysis of liver cancer for precision medicine

Liver cancer is that the third leading reason behind cancer-related death worldwide. Some thousands of carcinoma order are sequenced globally up to now and most of driver genes/mutations with high frequency are established in liver cancer, together with Wnt/β-catenin pathway, TP53/cell-cycle pathways, end maintenance, and body substance regulators. HBV integration into cancer-related genes is additionally a driver event in hepatocarcinogenesis. These genes are plagued by structural variants, copy-number alterations and virus integrations further as purpose mutations. Etiological factors of carcinoma is most understood among common cancers, like infectious disease, aflatoxin, alcohol, and metabolic diseases, and alteration signatures of willcer|cancer of the liver|liver disease|carcinoma} can offer proof of the association between specific etiological factors and mutational signatures. Molecular classifications supported corporal mutations profiles, ribonucleic acid expression profiles, and DNA methylation profiles are connected with patient prognosis. For exactness medication, many unjust mutations with solid proof like targets of multi-kinase inhibitors is determined in carcinoma, however there’s few molecular target medical aid up to now. it’s potential that rare unjust mutations in willcer|cancer of the liver|liver disease|carcinoma} can guide alternative specific molecular medical aid and immune therapy. [3]

CRISPR/Cas9 – An evolving biological tool kit for cancer biology and oncology

The development of biotechnology within the Seventies marked a brand new frontier in genome-editing technology. Gene-editing technologies have provided a embarrassment of advantages to the life sciences. The clustered frequently interspaced short palindromic repeats/CRISPR associated macromolecule nine (CRISPR/ Cas9) system could be a versatile technology that gives the flexibility to feature or take away polymer within the ordination in an exceedingly sequence-specific manner. Serious efforts are afoot to boost the potency of CRISPR/Cas9 targeting and so scale back off-target effects. Currently, numerous applications of CRISPR/Cas9 are employed in cancer biology and medicine to perform sturdy site-specific sequence piece of writing, thereby turning into a lot of helpful for biological and clinical applications. several variants and applications of CRISPR/Cas9 are being quickly developed. Experimental approaches that are supported CRISPR technology have created a awfully promising tool that’s cheap and easy for developing effective cancer medical specialty. This review discusses numerous applications of CRISPR-based gene-editing tools in medicine and potential future cancer therapies. [4]

Diagnostic Difficulty of Breast Lump- Revealed a Rare Entity

Lipoma could be a benign soft tissue neoplasm of animal tissue. Most of the time neoplasm is diagnosed supported clinical options. Histopathological confirmation required in bound cases. There are several subtypes of neoplasm. one among the rare variant of neoplasm is chondrolipoma. this can be a case report of fifty eight years recent feminine bestowed with lump within the right breast that contains a diagnostic problem. Later it had been diagnosed as chondrolipoma of breast. This case is according because of its rarity in breast. [5]

Reference

[1] Zhan, T., Rindtorff, N., Betge, J., Ebert, M.P. and Boutros, M., 2019, April. CRISPR/Cas9 for cancer research and therapy. In Seminars in cancer biology (Vol. 55, pp. 106-119). Academic Press. (Web Link)

[2] Mihanfar, A., Fattahi, A. and Nejabati, H.R., 2019. MicroRNA‐mediated drug resistance in ovarian cancer. Journal of cellular physiology, 234(4), pp.3180-3191. (Web Link)

[3] Nakagawa, H., Fujita, M. and Fujimoto, A., 2019, April. Genome sequencing analysis of liver cancer for precision medicine. In Seminars in cancer biology (Vol. 55, pp. 120-127). Academic Press. (Web Link)

[4] CRISPR/Cas9 – An evolving biological tool kit for cancer biology and oncology

Xueli Tian, Tingxuan Gu, Satyananda Patel, Ann M. Bode, Mee-Hyun Lee & Zigang Dong

npj Precision Oncologyvolume 3, Article number: 8 (2019) (Web Link)

[5] P. (2015) “Diagnostic Difficulty of Breast Lump- Revealed a Rare Entity”, Journal of Advances in Medical and Pharmaceutical Sciences, 4(4), pp. 1-3. doi: 10.9734/JAMPS/2015/20643. (Web Link)

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